当对两个核酸序列进行比对时，K-Estimator用来评估两者核苷酸替代数（趋异性），包括蛋白编码区与非蛋白编码区。使用Monte Carlo模拟估算可信区间。在分子进化研究中，评估两条核酸序列的被替换核苷酸数量是一个中心课题。精确量化这些数据将直接影响广泛应用于进化基因学的许多实验的可靠性。-When the two nucleic acid sequences, when compared, K-Estimator used to assess the number of nucleotide substitutions between the two (Divergent), including the protein coding region and non-protein coding region. Using Monte Carlo simulation estimates of confidence intervals. In molecular evolutionary studies to assess the two nucleic acid sequences is replaced by the number of nucleotides is a central issue. Accurate quantification of these data will directly affect the evolution of genetics is widely used in many experiments of reliability.