我们提出了一个控制适用于水或oilimmersion荧光显微镜和脂质双层囊泡相行为的应用定制设计室的温度。该装置是自包含的，携带方便，适合多用户镜设施。它提供了比任何商业设备更高的温度分辨率和稳定性，对阶毫度。我们证明了系统的实用性治疗的转换膜的研究。依赖于温度的相行为的模型膜系统thatdisplay液态有序lophase共存的液体无序ldphase是相关的理解在生物细胞质膜的异质性的存在，ubiquitouslytermed“脂筏。”2010美国物理研究所。-We present the design of a custom temperature-controlled chamber suitable for water or oilimmersion fluorescence microscopy and its application to phase behavior in lipid bilayer vesicles.The apparatus is self-contained and portable, suitable for multiuser microscopy facilities. It offers ahigher temperature resolution and stability than any comparable commercial apparatus, on the orderof millidegrees. We demonstrate the utility of the system in the study of miscibility transitions inmodel membranes. The temperature-dependent phase behavior of model membrane systems thatdisplay liquid-ordered Lophase coexistence with the liquid-disordered Ldphase is relevant tounderstanding the existence of heterogeneities in biological cell plasma membranes, ubiquitouslytermed “lipid rafts.”　 2010 American Institute of Physics. doi:10.1063/1.3483263